Part:BBa_K1725426:Design

J23101 + RBS + UirR + B0015

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 206
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 510
  

Design Notes

The regulator and J23101 were ligated to B0015 after restriction that was followed by an Azure-A-based gel extraction.

Source

The regulator comes from the genomic sequence of the unicellular cyanobacterium Synechocystis sp. PCC6803.